Irradiated Human PBMCs
Irradiated PBMCs cannot proliferate but are alive and retain their cellular processes, including antigen processing and presentation and production of cytokines and other soluble factors. Irradiated PBMCs can be used as stimulator cells for rapid PBMC culture expansion, as antigen-presenting cells to present antigen to T cells, as feeder cells to support the culture of target cells (e.g., CAR-T cells), or as stimulator cells in mixed lymphocyte reactions.
X-Ray irradiated PBMCs are a much safer alternative to using gamma rays from a Cesium 137 source as there is no radiologic material used, no production of radioactive waste, and no risk of radioactive contamination. X-Ray irradiated human PBMCs are also a better option than using Mitomycin C treated PBMCs, since Mitomycin C is toxic and requires extra handling steps that leads to PBMC loss.
Our irradiated peripheral blood mononuclear cells are available in 25M, 50M, and 100M vial sizes and are produced by exposing the PBMCs to an X-Ray source at a specific dose to create enough DNA damage to stop cell division, while maintaining high cellular viability.