Human M2 Macrophages

M2 macrophages are derived from purified CD14+ monocytes. CD14+ monocytes are positively selected via immunomagnetic beads and cultured in the presence of M-CSF for 10 days. The M2 phenotyping is confirmed by morphology and the expression of specific biomarkers monitored by flow cytometry. The cells are harvested after 10 days of in vitro culture.