This population contains >20% CD8 T cells specific to the WT-1 (Wilm’s Tumor gene product) peptide RMFPNAPYL (aa 126-134) presented by HLA-A*0201. WT-1 protein is overexpressed in leukemia and various solid cancers.
Donor has an allergy to kidney beans and seasonal allergies. She does not take any medications and does not smoke. She has the V/V genotype for FcgRIII alpha
B Lymphoblastoid Cell lines are derived by infection of PBMC with Epstein-Barr virus. The virus immortalizes B lymphocytes. It typically is not shed from cells but cultures should still be handled under Biosafety level 2 conditions.
Healthy donor taking no medications. She had osteosarcoma surgically removed in 2005.